The chromophore of phytochrome.
نویسندگان
چکیده
The chromophore of phytochrome, the plant photomorphogenic pigment, was cleaved from the associated protein. Chromatographic and spectral properties indicated that it was a bilitriene closely similar to but distinct from the chromophore of C-phycocyanin and allophycocyanin.
منابع مشابه
Differences in the physical properties of native and partially degraded phytochrome as probed by their differential sensitivity to permanganate oxidation.
The differential sensitivities to permanganate oxidation of the red and far-red forms of native phytochrome from Avena sativa L. cv Mulaga (isolated as Pfr from red-irradiated tissue) and of partially degraded phytochrome (isolated as Pr from nonirradiated tissue) were determined. The far-red absorbing form of partially degraded phytochrome was 5 times more sensitive than its red-absorbing form...
متن کاملSynthesis of phytochrome apoprotein and chromophore are not coupled obligatorily.
G Gardner and HL Gorton (1985 Plant Physiol 77: 540) demonstrated that gabaculine (5-amino-1,3-cyclohexadienylcarboxylic acid) inhibits the initial synthesis and resynthesis of spectrophotometrically detectable phytochrome in pea, maize, and oat. We show that the level of immuno-detectable phytochrome in pea is unaffected by the presence of gabaculine at a concentration that reduces spectrophot...
متن کاملIn vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore.
Phytochrome B (PhyB), one of the major photosensory chromoproteins in plants, mediates a variety of light-responsive developmental processes in a photoreversible manner. To analyze the structural requirements of the chromophore for the spectral properties of PhyB, we have designed and chemically synthesized 20 analogs of the linear tetrapyrrole (bilin) chromophore and reconstituted them with Ph...
متن کاملThe Arabidopsis photomorphogenic mutant hy1 is deficient in phytochrome chromophore biosynthesis as a result of a mutation in a plastid heme oxygenase.
The HY1 locus of Arabidopsis is necessary for phytochrome chromophore biosynthesis and is defined by mutants that show a long hypocotyl phenotype when grown in the light. We describe here the molecular cloning of the HY1 gene by using chromosome walking and mutant complementation. The product of the HY1 gene shows significant similarity to animal heme oxygenases and contains a possible transit ...
متن کاملTetranitromethane oxidation of phytochrome chromophore as a function of spectral form and molecular weight.
Tetranitromethane bleaches Avena phytochrome. The phytochrome (far-red absorbing form; Pfr) chromophore of 124 kilodalton (kD) phytochrome is oxidized 8 times more rapidly than the red absorbing form (Pr). Proteolysis of the 124 kD molecule to the extensively studied mixture of 118 and 114 kD polypeptides increases the rate of oxidation of Pfr 5-fold without affecting the rate of Pr oxidation. ...
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ورودعنوان ژورنال:
- Plant physiology
دوره 41 8 شماره
صفحات -
تاریخ انتشار 1966